液相色谱-三重四极杆质谱同时测定食品接触材料中双酚A、双酚F与双酚S的迁移量

Determination on Migration of Bisphenol A,Bisphenol F and Bisphenol S in Food Contact Materials by Liquid Chromatography-Tandem Mass Spectrometry

采用液相色谱-三重四极杆质谱(LC-MS/MS)建立了食品接触材料中双酚A、双酚F和双酚S化合物的同时测定方法。双酚A、双酚F和双酚S在样品中通过与食品模拟物在一定温度、时间接触的状态下迁移到食品模拟物中,以C18色谱柱进行分离,甲醇-氨水为流动相洗脱,采用电喷雾离子源(ESI),多反应监测(MRM)负离子模式进行扫描,同位素内标法定量。结果表明,双酚A、双酚F和双酚S的色谱分离良好,双酚A和双酚F在1~50μg/L质量浓度范围内与其峰面积均呈线性关系,BPS在0.1~50μg/L质量浓度范围内与其峰面积呈线性关系。BPA和BPF的最低检出限为0.3μg/L,BPS的最低检出限为0.05μg/L。加标回收率为83.6%~102.6%,相对标准偏差(RSD)为3.5%~8.9%。该方法快速可靠、准确简便,适用于食品接触材料中双酚A、双酚F和双酚S化合物的检测。

A high performance liquid chromatography-tandem mass spectarometry was established for the determination on migration amounts of bisphenol A,bisphenol F and bisphenol S in food contact materials.Bisphenol A,bisphenol F and bisphenol S were transfered into the food simulants in the sample contacted at a certain temperature and time.The separation was performed using a C 18 chromatographic column with methanol-ammonia as mobile phase.Under the electrospray ionization(ESI)mode,bisphenol A,bisphenol F and bisphenol S were detected in a multiple-reaction monitoring mode(MRM),and quantified by the external standard method.The method indicated a perfect performance in chromatographic separation.The calibration curves of bisphenol A and bisphenol F were linear in the range of 1-50μg/L,while the calibration curve of bisphenol S was linear in the range of 0.1-50μg/L.The limits of detection were 0.3μg/L for bisphenol A and bisphenol F,and 0.05μg/L for bisphenol S.The recoveries ranged from 83.6%to 102.6%,with relative standard deviations(RSDs)of 3.5%-8.9%.This method was rapid,accurate,precise and convenient,and was appropriate for the determination of bisphenol A,bisphenol F and bisphenol S in food contact materials.