顶空气相色谱法测定食品添加剂中15种有机溶剂残留量

Determination of 15 Organic Solvents Residues in Food Additives by Headspace-Gas Chromatography

建立了顶空气相色谱法同时测定食品添加剂中甲苯、甲醇、正己烷等15种有机溶剂残留量的分析方法。选择N,N-二甲基甲酰胺对不同食品添加剂中的有机溶剂进行提取,将样液转移至顶空瓶中密封,顶空进样测定。对提取溶剂、提取方式、平衡时间和平衡温度等进行了优化。结果表明,15种有机溶剂分离良好,各残留溶剂在相应浓度范围内呈良好的线性关系,相关系数不小于0.999 8,定量下限为10.0 mg/kg(三氯甲烷和吡啶为20.0 mg/kg),回收率为90.6%~109.1%,相对标准偏差为0.1%~7.2%。该方法灵敏度高,重现性好,适用于食品添加剂中甲苯、甲醇、正己烷等15种残留溶剂的检测分析。

An analytical method for the simultaneous determination of 15 kinds of organic solvents residues in food additives based on headspace-gas chromatography was established.The residual organic solvents in different food additives were extracted with N,N-dimethylformamide using centrifuge tubes.To achieve the optimum conditions for the analysis,several parameters including the extraction solvents,extraction method,equilibrium temperature and equilibrium time were optimized.The results showed that there existed good linearities for the solvent residues in the respective concentration ranges with their correlation coefficients no less than 0.999 8.The limits of quantification for the target compounds were 10.0 mg/kg except that those for pyridine and chloroform were 20.0 mg/kg.The recoveries for 15 organic solvents residues were in the range of 90.6%-109.1%with relative standard deviations(RSDs)of 0.1%-7.2%.This method is sensitive and accurate,and is suitable for the determination of organic solvents residues in food additives.