摘要
运用微卫星分子标记研究洞庭湖东方田鼠(Microtus fortis calamorum)的多父权行为。利用筛选获得的6对微卫星引物对7只母鼠及其胎崽DNA进行PCR扩增,PCR产物经凝胶电泳和银染后分析,结果表明2只母鼠所怀的胎崽明显具有2个以上的父权现象,其他母鼠所怀的胎崽至少拥有一个父权。研究结果显示东方田鼠存在多父权的婚配制度。
A method of microsatellite marker was employed to confirm multiple paternity in the Yangtze vole(Microtus fortis calamorum)distributed in Dongting Lake.Six pairs of primers were screened for better PCR products.After PCR,gel electrophoresis and silver staining,the alleles of seven mothers and their offspring were analyzed.The results showed that two litters were sired by more than two male voles,respectively.Mating behavior of the vole was characterized with multiple paternity.
作者
郭宗明
王勇
郭聪
GUO Zong-ming;WANG Yong;GUO Cong(Yantai Nanshan University,Yantai 265713;College of Life Sciences,Sichuan University, Chengdu 610064;Key Laboratory Agro-ecological Processes of Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2018年第1期56-59,共4页
Journal of Biology
基金
国家基础研究项目(973计划)(2007CB109106)
中科院创新研究项目(KSCX2-YW-N-06)
关键词
东方田鼠
婚配行为
微卫星
多父权
the Yangtze vole
mating behavior
microsatellite marker
multiple paternity
