雄性中华狼蛛毒素多肽的分离及鉴定

Isolation and identification of peptides derived from venom in male Lycosa sinensis

采用反向高效液相色谱对雄性中华狼蛛粗毒进行分离,再用MALDI–TOF–TOF质谱进行相对分子质量测定。结果表明:从雄性中华狼蛛粗毒中一共鉴定到195种毒素多肽组分,这些毒素多肽的相对分子质量为1 000~10 000,其中大多数多肽的相对分子质量为1 000~3 500和4 500~7 500。这些毒素多肽的相对分子质量的分布特征与已报道的其他蜘蛛的不同,这可能是因为不同种类蜘蛛的毒素成分具有不同的多样性特点。

Peptides in crude venom from male Lycosa sinensis were isolated by means of reversed–phase high–performance liquid chromatography,and their relative mass in eluted fractions were analyzed through MALDI– TOF–TOF mass spectrometry.In total,195 peptides were detected out with the relative mass ranged from 1 000 to 10 000.The vast majority of these peptide mass fell in the ranges of 1 000–3 500 and 4 500–7 500,respectively.Its molecular weight distribution was far dissimilar from those of other spiders reported,which is probably because venom components might vary with spider species.