大豆异黄酮对去卵巢大白鼠垂体雌激素受体和白细胞介素–2表达的影响

Effects of soybean isoflavone on protein and m RNA expression of ERα and IL–2 in pituitary of ovariectomized rats

为探讨大豆异黄酮(SIF)对雌激素受体(ERα)和白细胞介素–2(IL–2)在去卵巢大鼠垂体各叶中表达的影响,对去卵巢大鼠皮下注射不同剂量的SIF(分别皮下注射1.5、1.0、0.5 mg/kg的SIF)和无水乙醇,另设假手术组,注射等量无水乙醇。待SIF处理至第2、6周时,于各组随机选取5只大鼠进行剖杀,分别对大鼠垂体中ERα和IL–2的蛋白及m RNA表达变化进行研究。结果显示,大鼠去除卵巢后,垂体各叶中ERα和IL–2的蛋白及m RNA表达强度和阳性细胞数均显著下降,补充SIF后有不同程度升高,其中高剂量SIF作用6周后,各指标的表达基本恢复至假手术组水平,其他各组随SIF的剂量增加及作用延长也有部分恢复,表明大豆异黄酮对去卵巢大鼠垂体各叶内ERα和IL–2蛋白及m RNA的表达有促进作用,呈现时间和剂量的依赖性。

To explore the effects of soybean isoflavone(SIF)on expression of estrogen receptor alpha(ERα)and interleukin–2(IL–2)protein as well as mRNA in the pituitary of ovariectomized rat,forty healthy female rats were ovariectomized,and then respectively supplied with SIF at high dosage(1.5 mg/kg),middle dosage(1.0 mg/kg),low dosage(0.5 mg/kg)and solvent agent by mean of hypodermic injection,at the same time,sham operation group was injected at the same amount of solvent agent.After treatments of the second and sixth week,5 rats out of each group were randomly selected for the study.The expression and distribution of ERα,IL–2 protein and mRNA in the pituitary were studied using technique of immunohistochemical(IHC)and in situ histo–hybridization method(ISHH).The results showed that the general tendency of intensity and the number of positive cells of ERαand IL–2 in pituitary of ovariectomized group rats were significantly decreased with time after ovariectomized.While,in ovariectomized rats treatment with SIF,the general tendency of the intensity and the number of ERαand IL–2 positive cells were increased,especially at high doses of soy isoflavones for six weeks,the intensity and quantity of these products were even reached to the level of that in the sham–operation group,while,in other groups,only part were recovered with SIF dose and time increase.The above results indicated that SIF could upregulate the expression of ERαand IL–2 protein as well as mRNA in the pituitary ovariectomized rat,and it showed a time and dose dependent manner.