口蹄疫病毒细胞培养技术研究进展

Advance in Cell Culture Techniques of Foot-and-Mouth Disease Virus

口蹄疫病毒可以在原代细胞和传代细胞上增殖,传统培养采用贴壁培养方式,随着微载体技术、无血清培养基研制和悬浮培养技术的发展,无血清培养法和生物反应器自动化设施提高了口蹄疫病毒含量,降低了生产成本,但直接影响了动物免疫保护效力。随着基因编辑技术的发展,病毒种子毒、细胞株和细胞微环境研究突飞猛进,这为口蹄疫疫苗制备提供了新的技术支撑。论文介绍了口蹄疫病毒在原代细胞、传代细胞的增殖方式和营养特点,以及贴壁培养和悬浮培养的优缺点,为口蹄疫疫苗的研发和生产提供技术参考。

Foot-and-mouth disease virus(FMDV)can multiplicate in primary cells and passage cells.With the development of microcarrier cell culture,serum-free medium and subculture of suspension cells,these advancements may elevate the titres of FMDV,reduce the costs and improve the efficacy of FMDV vaccine in animals.Virus seeds,cell lines and celluar microenvironments are changing greatly as the development of gene-editing technologies,which support FMD vaccine study.In this review,we introduced the characteristics of FMDV multiplication and nutrition in primary and passage cells as well as the pros and cons between cell adhesion and suspension culture techniques.Moreover,this review also shed light on the development and production of FMD vaccine.