响应面法优化超声波辅助北苍术多酚提取工艺及其DPPH自由基清除能力研究

Optimization of ultrasonic assisted extraction of polyphenols from Atractylodes chinensis(DC.) Koidz. using response surface methodology and evaluation of its antioxidant activity

探讨北苍术多酚的最佳提取工艺,并评价北苍术多酚的DPPH自由基清除能力。采用超声波辅助提取方法,以乙醇浓度、液料比和超声时间进行单因素实验;以北苍术多酚提取量为响应值,进一步采用响应面法优化北苍术多酚的提取工艺条件,以抗坏血酸为对照,通过测定北苍术多酚清除DPPH自由基的能力来评价其抗氧化活性。实验结果表明,北苍术多酚最优提取工艺条件为:乙醇浓度74%(质量分数),液料比25mL/g,超声时间37 min,在此条件下北苍术多酚提取量达到(11.67±0.15)mg/g,与预测值相对误差为1.97%。该优化工艺可行;优化的北苍术多酚提取工艺科学、合理,且提取的北苍术多酚具有较好的DPPH自由基清除能力。

To optimize the extraction technology of polyphenols from Atractylodes chinensis(DC.)Koidz.and evaluate its antioxidant activity.The ultrasonic assisted extraction method was carried out.The effect of ethanol concentration,liquid-solid ratio and ultrasonic time on the yield of polyphenols from Atractylodes chinensis(DC.)Koidz.were investigated by single-factor experiment.Response surface methodology was then used to optimize the extraction technology conditions of polyphenols from Atractylodes chinensis(DC.)Koidz.The antioxidant activity was evaluated by DPPH free radicals scavenging method taking ascorbic acid as the control.The results showed that the optimal extraction technology of polyphenols was as follows:ethanol concentration 74%,liquid-solid ratio 25:1 mL/g and ultrasonic time 37 min,and the experimentally measured yield of polyphenols was(11.67±0.15)mg/g,with a relative error of only 1.97%compared to the model-predicted value.Thus,the conditions are optimal for polyphenols extraction.The IC 50 of polyphenols was 2.10 mg/mL in DPPH radical scavenging,which was lower than that of scorbic acid.The optimization of ultrasonic assisted extraction of polyphenols from Atractylodes chinensis(DC.)Koidz.was reasonable and scientific,and the polyphenols from Atractylodes chinensis(DC.)Koidz.had antioxidant activity.