MicroRNA-204在非小细胞肺癌患者组织中的表达及对癌细胞增殖和凋亡的影响

miRNA-204 expression in non-small cell lung cancer tissue and its effect on cancer cell proliferation and apoptosis

目的观察microRNA-204(miR-204)在非小细胞肺癌(NSCLC)患者体内的表达水平及对癌细胞增殖的影响,探讨miR-204在非小细胞肺癌中的临床意义及可能分子机制。方法实时荧光定量聚合酶链反应(qRT-PCR)检测miR-204在肺癌及癌旁组织中的表达情况;用化学合成的miR-204模拟物和抑制物转染人非小细胞肺癌A-549肺癌细胞,CCK-8法检测细胞增殖的情况,流式检测细胞凋亡情况,qRT-PCR、Western blot法分别检测Bcl-2的mRNA和蛋白表达。结果 miR-204在肺癌组织中表达水平与对应癌旁组织比较,差异有统计学意义(P<0.05),肺癌组织低于癌旁组织;肺癌组织中miR-204低表达与分期、肿瘤大小、肿瘤淋巴结转移相关(P<0.05);miR-204可抑制A-549细胞的增殖,促进细胞凋亡,并下调Bcl-2的mRNA与蛋白表达水平。结论 miR-204在非小细胞肺癌组织中表达下调并与肺癌恶性临床病理特征有关,miR-204可能通过调节Bcl-2的表达从而影响非小细胞肺癌细胞的增殖及凋亡。

Objective To observe the expression of miRNA-204 in the lung tissue of non-small cell lung cancer(NSCLC)patients and its effect on lung cancer cell proliferation and apoptosis.Methods qRT-PCR was used to detect the expression of miRNA-204 in the lung cancer and peri-cancerous tissues.After A-549 cells were interfered with miRNA-204 mimics,the cell proliferation was observed using CCK-8,cell apoptosis was observed by flow cytometry,and the mRNA and protein levels of Bcl-2 were detected by qRT-PCR and Western blot respectively.Results The expression of miRNA-204 in the NSCLC tissues was significantly lower than that in the peri-cancerous tissues(P<0.05).And the low-expression of miRNA-204 in the NSCLC tissues was associated with tumor size and lymph node metastasis(P<0.05).After interference of miRNA-204 mimics in the A-549 cells,the cell proliferation was reduced and the cell apoptosis was increased,and the mRNA and protein levels of Bcl-2 were decreased(P<0.05).Conclusions The expression of miRNA-204 in the NSCLC tissues is decreased and related to the malignant clinicopathological features of lung cancer.miRNA-204 may affect the proliferation and apoptosis of non-small cell lung cancer cells by regulating Bcl-2 expression.