当药黄素对H_2O_2诱导PC12细胞损伤的保护作用

Protective Effects of Swertisin on Oxidative Damage of PC12 Cells Induced by H_2O_2

研究当药黄素在H_2O_2诱导PC12细胞损伤中的作用。建立H_2O_2诱导的PC12细胞损伤模型,采用MTT法测定细胞活力,比色法测定细胞MDA和上清液LDH含量,以及SOD、CAT和GSH-Px酶活力,采用DCFH-DA荧光染色测定细胞ROS含量,JC-1染色测定细胞线粒体膜电位。当药黄素能够提高H_2O_2诱导损伤的PC12细胞的细胞活力,增加细胞内SOD、CAT和GSH-Px活力,降低MDA、LDH含量,抑制细胞内ROS增加,稳定细胞线粒体膜电位。当药黄素对H_2O_2诱导的PC12细胞损伤有保护作用。

This study was conducted to investigate the protective effect of swertisin on damage PC12 cells induced by H2O2,as well as its primary mechanism.The cell viability was measured by MTT assay.The activity of SOD,CAT and GSH Px,the level of were measured by the colorimetric method.The cell reactive oxygen species(ROS)was detected by the reactive oxygen test kit.The cell mitochondrial membrane potential was detected by the mitochondrial membrane potential detection kit(JC-1).The investigation showed that swertisin can improve cell viability in damage PC12 cells induced by H2O2,inhibit the releasing of MDA and LDH,enhance SOD,CAT and GSH-Px activities,inhibit intracellular ROS increase,and stable mitochondrial membrane potential.The results demonstrated that swertisin can suppress the oxidative damage in PC12 cells induced by H2O2 in multiple levels,which play a protective role in nerve cells.