油茶籽粕中茶皂素的优化提取及抑菌活性的研究

Extraction and Bacteriostasis Effect of Tea Saponin in Oil-tea Camellia Seed Cake

以油茶籽粕为原料,采取超声波辅助乙醇提取法,考察了料液比、浸提时间、超声时间、乙醇体积分数对茶皂素得率的影响,并用响应面优化实验设计得出最优提取条件,优化条件为:料液比1∶7(m/V)、浸提时间93min、超声时间36 min、乙醇体积分数55%,在该条件下茶皂素得率为14.708%。依照此优化方案进行验证实验,结果显示模型可靠性较高,可以较好的预测实际情况。同时茶皂素抑菌效果表明茶皂素对金黄色葡萄球菌有较好的抑菌效果,而对大肠杆菌没有抑菌效果。

Using camellia seed cake as raw material,the effect of solid to liquid ratio,extraction time,ultrasonic time and volume fraction of ethanol on the yield of tea saponin was studied by using ultrasonic assisted ethanol extraction method.The optimal extraction was carried out using the response surface optimization experiment.The optimal conditions were as follows∶solid to liquid ratio was 1∶7(m/V),extraction time 93 min,ultrasonic time 36 min,volume fraction of ethanol 55%,and the yield of tea saponin was 14.708%under these conditions.According to this optimization scheme,the testing results showed that the model had high reliability and can predict the actual situation.Meanwhile,the results of bacteriostatic effect showed that tea saponin had better bacteriostasis effect on Staphylococcus aureus,but had no bacteriostasis effect on Escherichia coli.