多重PCR测序揭示浙蒲系列瓠瓜优异瓜形与鲜味的基因型基础

Genotypic basis of elite fruit shape and umami taste traits in Zhepu bottle gourds as revealed by multiplex PCR

外观品质和风味品质是瓠瓜育种的重要指标。以浙蒲系列瓠瓜品种为材料,利用多重PCR的方法,检测已知的16个鲜味相关SNPs和37个瓜形相关SNPs。研究表明,浙蒲6号和浙蒲8号均含有4个谷氨酸含量增效等位变异和11个瓜型等位变异;浙蒲1号含有6个谷氨酸含量增效等位变异和1个瓜形等位变异,浙蒲9号含有5个谷氨酸含量增效等位变异和2个瓜形等位变异。试验结果初步揭示了浙蒲系列瓠瓜品种优异外形与鲜味品质的基因型基础,为利用分子标记进一步开展瓠瓜优美瓜形、鲜味品质与其他优良性状的的聚合育种奠定基础。

Breeding for cultivars with elite fruit shape and umami taste traits is an important objective of bottle gourd breeders.In this study,we examined the genotypes of 4 Zhepu bottle gourd cultivars at 16 known SNPs associated with umami taste and 37 SNPs for fruit shape by using a multiplex PCR technology.We found Zhepu No.1 has 6 alleles improving free Glu content and 1 allele for elite fruit shape,Zhepu No.9 harbored 5 alleles improving free Glu content and 2 alleles for elite fruit shape,while Zhepu No.6 and Zhepu No.8 both contained 4 free Glu content-improving alleles and 11 elite fruit shape alleles.Our study preliminarily reveals the genotypic basis of the excellent fruit shape and peculiar umami taste in several Zhepu bottle gourds,which will facilitate stacking these traits with other favored agronomical traits by marker assisted selection.