苦瓜α-苦瓜素基因启动子克隆与单倍型分析

Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

为探明α-苦瓜素基因的表达调控规律,从苦瓜种质Y5中克隆了α-苦瓜素基因上游包括起始密码子在内的1 620 bp序列,选取转录起始位点上游1 500 bp序列,利用Plant CARE在线启动子预测工具进行分析。结果表明,α-苦瓜素启动子除了含有TATA-box和CAAT-box等核心元件外,还含有光响应元件、赤霉素响应元件、热胁迫响应元件、干旱应答元件、水杨酸应答元件、茉莉酸应答元件等。以28份苦瓜种质为材料,分析了α-苦瓜素启动子区域SNP和In Del分布情况,共发现24个SNP位点和1个In Del,28份苦瓜种质资源共存在4种单倍型。3个SNP位于转录因子结合位点,可能对α-苦瓜素基因的表达调控有重要作用。

In order to explore the expression regulation ofα-momorcharin gene,the upstream sequence,1 620 bp in length including start codon ofα-momorcharin gene was cloned in bitter gourd(Y5).The 1 500 bp upstream of the transcription start site was selected for analysis based on PlantCARE promoter prediction tools.The results showed that in addition to containing the core elements of TATA-box and CAAT-box,theα-momorcharin promoter also contained light response elements,gibberellin response element,heat stress response element,drought response element,salicylic acid response element,jasmonic acid response element etc.Taking 28 bitter gourd germplasm as materials,the SNP and InDel distribution in promoter region ofα-momorcharin was analyzed.Twenty-four SNP loci and 1 InDel were found.Four haplotypes were identified inα-momorcharin gene promoter through SNP and InDel grouping.Three SNPs were located in transcription factor binding sites,which may play important roles in regulating the expression ofα-momorcharin gene.