不同年份橡胶树未受粉胚珠愈伤组织诱导的研究

Study on Induction of Rubber Tree Unpollinated Ovules Callus in Different Years

为探明不同年份橡胶树未授粉胚珠愈伤组织的诱导情况,以云研73-477、云研77-2、云研77-4、热研8-79及RRII105的未授粉胚珠为外植体,于2013-2015年对橡胶树未授粉胚珠愈伤组织的诱导开展研究。结果表明:愈伤组织的诱导率在品种间的差异在3年里均达到极显著水平(P<0.01),且普遍偏低,总体均值仅为26.07%;云研73-477胚珠的愈伤组织诱导率在年度间差异均达到显著水平(P<0.05),另外4个品种的差异达到极显著水平(P<0.01)。从3年的愈伤组织诱导率来看,热研8-79最高,其年份均值达到51.57%,变幅为49.72%~53.62%;其次是云研73-477,其年份均值为28.50%,变幅为26.00%~31.03%;最小的为RRII105,其年份均值仅为8.92%,变幅为5.88%~13.40%。

In order to clarify the difference of callus induction of rubber tree unfertilized ovules in different years.Studies on induction of rubber tree unpollinated ovules callus were carried out in the three years from 2013 to 2015 by using unpollinated ovules of Yunyan 73-477,Yunyan 77-2,Yunyan 77-4,Reyan 8-79 and RRII105 as explants.The results showed that:(1)The varietal difference of callus induction rates reached an extremely significant level(P<0.01)in the years from 2013 to 2015,and the rates were generally low with an average value of 26.07%only.(2)The differences during different years in induction rates of Yunyan 73-477’s ovules callus were at a significant level(P<0.05),while the differences of other four varieties reached a very significant level(P<0.01).Reyan 8-79 had the highest induction rate in three years,its population mean reached to 51.57%,and the amplitude was 49.72%~53.62%,followed by Yunyan 73-477,which population mean was 28.50%,and amplitudes was 26.00%~31.03%;Whereas the induction rates of RRII105 was the lowest,with which population mean was 8.92%,and amplitude was 5.88%~13.40%.