奥美拉唑对胃癌上皮细胞株、幽门螺杆菌及空泡毒素作用的体外试验

Effect of omeprazol on growth of SGC-7901 cell line, Helicobacter pylori and VacA toxicity in vitro

目的 :通过奥美拉唑对胃癌上皮细胞、幽门螺杆菌 (Hp)菌株生长及空泡毒素 (VacA)毒性的影响 ,探讨其临床应用价值。方法 :应用不同浓度奥美拉唑和细胞、细菌及VacA共孵育方法观察细胞、细菌的生长状况及空泡毒性的变化。结果 :(1)当奥美拉唑浓度 >10 0mg·L- 1时 ,细胞 10 0 %死亡 ;低于4 0mg·L- 1时 ,光镜下未见上皮细胞的形态学改变。(2 )当奥美拉唑≥ 10 0mg·L- 1时 ,细菌生长完全被抑制 ;低于 30mg·L- 1,细菌的生长不受影响。 (3)低浓度奥美拉唑 (10～ 4 0mg·L- 1)能部分抑制Va cA毒素对SGC 790 1的空泡作用 ,但不抑制毒素对HeLa细胞的空泡毒性作用。结论 :抑制Hp生长所需的奥美拉唑浓度对胃上皮细胞也有损伤作用 ,对空泡毒素无显著的抑制作用 ,临床上只能应用低剂量奥美拉唑 ,通过抑制胃酸分泌治疗酸相关疾病 。

AIM: To probe the clinical value of omeprazole through observing the effect of omeprazole on the growth of SGC-7901 cell line and Helicobacter pylori(Hp), and VacA activity. METHODS: The growth of SGC-7901 cell line, Hp and vacuolating cytotoxin of VacA were observed using coincubation of cell line or Hp or VacA with omeprazole. RESULTS: (1) All of cells died when omeprazole of >100 mg·L -1 was added in culture medium; the structures of cells under microscopy did not change when omeprazole <40 mg·L -1 in medium. (2) The growth of Hp was completely inhibited when omeprazole of ≥100 mg·L -1 was added in culture medium; omeprazole <30 mg·L -1 could not inhibit the growth of Hp. (3) Omeprazole of 10-40 mg·L -1 could partly inhibit vacuolating cytotoxin of culture supernatant of Hp on SGC-7901 cell line, but did not on HeLa cells. CONCLUSION: Omeprazol can inhibit the grouwth of Hp as well as damage SGC-7901 cells. Omeprazol partly inhibit vacuolating cytotoxin of VacA. In clinic practise, omeprazole can not be applied to kill Hp. It can just inhibit secretion of gastric acid.