西藏一种芥菜型油菜PGIP基因的克隆与序列分析

Cloning and bioinformatic analysis of PGIP gene from Brassica junceain Tibet

防治油菜菌核病的有效方法是培育抗菌核病的油菜品种。多聚半乳糖醛酸酶阻遏蛋白(polygalacturonase inhibiting protein,PGIP),是植物体内一种重要的抗真菌蛋白。本研究根据NCBI中发表的PGIP基因序列设计引物,从芥菜型油菜‘藏油6号’中克隆得到全长1 048bp的PGIP基因序列。其核酸序列与NCBI数据库中登录的PGIP基因序列同源性为99%。该序列翻译后得到的氨基酸序列与NCBI上公布的油菜PGIP序列同源性为99%。该蛋白质序列中包含8个亮氨酸重复区(leucine-rich repeat,LRR)。过去研究结果显示芥菜型油菜比甘蓝型、白菜型油菜对菌核病的抗性普遍较高,但是未能搞清楚具体的原因,只是提出抗菌核病基因主要分布在芥菜型油菜中,其次是甘蓝型油菜中。通过研究证实了芥菜型油菜上PGIP基因具有保守性、疏水性,而且结构稳定,具有多个信号肽,能够高效地发挥其抗核盘菌的功能。今后在油菜育种上应利用芥菜型油菜的优势,发挥其抗病育种的潜力。

The best way to prevent Sclerotinia is to cultivate varieties against Sclerotinia.PGIP is an important anti-Sclerotinia protein.The full-length of PGIP CDS(1 048 bp)was cloned from Brassica juncea No.6 in Tibet based on sequence homology,showing 99%similarity with PGIP from B.napus.The protein sequence contained 8 LRRs(leucine-rich repeats).Previous studies showed that B.juncea had higher resistance to S.sclerotiorum than B.napus and B.campestris,but the underlying mechanism was unknown,though it was known that S.sclerotiorum genes were mainly distributed in B.juncea.The PGIP gene in B.juncea is conservative,hydrophobic,and stable in structure with several signal peptides,which has the potential for resistance to S.sclerotiorum and disease resistance breeding.