骨髓间充质干细胞移植对周围神经损伤后施旺细胞的影响

Effects of BMSCs Transplantation on Schwann Cells after Peripheral Nerve Injury

目的探讨骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)移植对周围神经损伤后宿主施旺细胞的保护作用。方法分离、培养SD大鼠的BMSCs,制作坐骨神经横断损伤模型,将第4代BMSCs植入坐骨神经损伤远侧段,HE染色和免疫荧光染色检测施旺细胞的形态和数量。结果 HE染色和免疫荧光染色均显示,BMSCs植入后,损伤坐骨神经的施旺细胞数量增多,细胞结构更加清晰完整,排列更加整齐有序。结论 BMSCs植入可以促进坐骨神经损伤后施旺细胞的存活或再生,可作为治疗周围神经系统损伤的干细胞移植的种子细胞。

Objective To explore the effects of bone marrow mesenchymal stem cells(BMSCs)transplantation on Schwann cells after peripheral nerve injury.Methods BMSCs were isolated from SD rats and cultured.Injury model of the sciatic nerve cross-sectional was established,and BMSCs were injected into the distal of injured sciatic nerve.HE staining and immunofluorescence staining were used to detect the morphology and quantity of Schwann cells.Results According to HE staining and immunofluorescence staining,after BMSCs was implanted,the number of Schwann cells in sciatic nerve increased,the cell structure was more clear and complete,and the arrangement was more orderly.Conclusion BMSCs transplantation can protect Schwann cells after sciatic nerve injury.BMSCs may be a candidate in cell transplantation therapy for peripheral nerve injury.