衍生化法测定蓝莓果汁饮料中花色苷含量

Determination of the content of Anthocyan ins of blueberry juice drinkderivatization method

柱前衍生,高效液相色谱法测定蓝莓果汁饮料中花色苷的含量。在温度为353K条件下,蓝莓果汁饮料与25%盐酸/甲醇溶液(V∶V=1∶1)反应1h,经过0.45μm微孔滤膜过滤后,采用高效液相色谱配二极管阵列检测器检测,以氯化矢车菊素为参照,外标法计算含量。采用Agilent HC-C18柱(250mm×4.6 mm,5μm),流动相A:0.4%三氟乙酸(TFA)水溶液,流动相B:0.4%TFA/乙腈溶液,梯度洗脱,流速1m L·min-1,进样体积20μL,柱温35℃,检测波长为190~800 nm,定量波长520nm。平均加标回收率为94.8%~98.0%,RSD为2.83%,检出限为0.008μg·m L-1。该方法简单、快速、成本低,适用于蓝莓果汁饮料中花色苷含量的测定。

Pre column derivatization,Determination of content of Anthocyanins of blueberry juice beverage by HPLC.When the temperature is under 353K conditions,Blueberry juice and 25%HCl/methanol solution(V∶V=1∶1)reaction of 1h,After 045μm microporous membrane filte,Using high performance liquid chromatography with diode array detector,In order to cyanidin chloride as reference,The content was calculated by external standard method.Conditions Using Agilent HC-C18 column(250mm×4.6 mm,5μm),The A of the mobile phase:04%three trifluoroacetic acid(TFA)aqueous solution,The B of the mobile phase:04%TFA/acetonitrile solution,Gradient elution,The flow rate was 1 mL·min-1,injection volume was 20μL,column temperature was 35℃,the detection wavelength was 190~800nm,quantitative wavelength was 520nm.Result The average recoveries for 94.8%~98.0%,2.83%for RSD,The detection limit is 0.008μg·mL-1.Conclusion The method is simple,rapid,low cost,Suitable for de-termination of content of anthocyanins from blueberry juice.