p57kip2和p27kip1在水泡状胎块中的表达及对比研究

The expression and comparison of p57kip2 and p27kip1 in hydatidiform moles

目的观察父源性印迹基因p57kip2和细胞周期负性调控因子p27kip1在不同类型水泡状胎块中的表达情况,探讨p57kip2和p27kip1在水泡状胎块中的表达及两者对于鉴别不同类型水泡状胎块的意义。方法采用免疫组织化学染色方法,检测30例完全性水泡状胎块、86例部分性水泡状胎块及30例正常绒毛组织中p57kip2和p27kip1的表达情况,分析p57kip2和p27kip1在两种不同类型水泡状胎块中的表达及两者之间的差异和相关性。结果 p57kip2和p27kip1在完全性水泡状胎块组的表达均低于部分性水泡状胎块组和正常流产绒毛组,差异有统计学意义(P<0.05),p57kip2和p27kip1在完全性水泡状胎块中表达呈正相关(r=0.750,P<0.01),在部分性水泡状胎块中表达呈负相关(r=-1.000,P<0.01)。结论 p57kip2和p27kip1在完全性水泡状胎块和部分性水泡状胎块中的表达差异对鉴别不同临床类型的水泡状胎块有一定指导价值,联合检测p57kip2和p27kip1可以对水泡状胎块的诊断和分型起到有效的辅助作用。

Objective To observe the level of imprinting gene p57kip2 and p27kip1 expression in different hydatidiform moles.To investigate the value of combined detection of p57kip2 and p27kip1 in diagnosis and differential diagnosis of hydatidiform moles.Methods We examined the immunohistochemical staining of p57kip2 and p27kip1 in 30 cases of complete hydatidiform moles and 86 cases of partial hydatidiform moles and 30 cases normal placenta,and also analyze the differences and correlation between the two genes of p57kip2 and p27kip1 in two different patterns of hydatidiform moles.Results The rate of expression of p57kip2 and p27kip1 in complete hydatidiform moles was obviously lower than that in partial hydatidiform moles and normal placenta.There were significant differences in the expression of p57kip2 and p27kip1 among complete hydatidiform moles,partial hydatidiform moles and normal placenta(P<0.05).p57kip2 had positive correlation with p27kip1 in complete hydatidiform moles(r=0.750,P<0.01),meanwhile there was negative correlation between p57kip2 and p27kip1 in partial hydatidiform moles(r=-1.000,P<0.01).Conclusion The differential expression of p57kip2 and p27kip1 in complete hydatidiform moles and partial hydatidiform moles can be of certain value in the differential diagnosis of hydatidiform moles.Meanwhile,the combined methed is useful to the identification and classification of hydatidiform moles.