DLBCL患者经GM-CSF治疗后外周血单个核细胞及树突状细胞变化研究

Evaluation of monocytes and dendritic cells changes in peripheral blood of diffuse large B-cell lymphoma patients before and after GM-CSF treatment

目的研究弥散性大B细胞淋巴瘤(DLBCL)患者经GM-CSF治疗后,外周血单核细胞、树突状细胞(DC)、髓系树突状细胞(mDC)以及浆系树突状细胞(pDC)的变化情况。方法 2014年10月至2016年2月入住该院的33例DLBCL患者静脉注射GM-CSF进行治疗,每周注射1次GM-CSF,每周为1个疗程,共治疗3个疗程。每次注射前,静脉采血3mL,采血后24h内用流式细胞仪对外周血单核细胞和DC进行检测。检测结果用SPSS17.0软件进行统计分析。结果 33例患者GM-CSF治疗前单核细胞水平为(9.85±2.31)%,3个疗程治疗后为(3.67±0.78)%,差异有统计学意义(P<0.01)。DC水平治疗前为(0.24±0.04)%,治疗后为(0.58±0.13)%,差异有统计学意义(P<0.01)。其中,mDC治疗水平为(0.11±0.02)%,低于治疗后的(0.36±0.08)%(P<0.01);pDC治疗前、后比较差异无统计学意义(P>0.05)。结论 DLBCL患者经GMCSF治疗3个疗程后,单核细胞明显减少,DC显著增加,其中mDC显著增加,pDC无明显变化,即GM-CSF治疗激活了DLBCL患者的抗肿瘤免疫。

Objective To evaluate the changes of monocytes,dendritic cells(DC),myeloid dendritic cells(mDC)and plasma dendritic cells(pDC)of peripheral blood in diffuse large B-cell lymphoma(DLBCL)patients after 3 courses of GM-CSF treatment.Methods 33 patients diagnosed with DLBCL in the hospital from October 2015 to February 2016 were enrolled in the study.All the patients were treated with GM-CSF for 3 courses.Before each course,3 mL venous blood samples were extracted and the monocytes,dendritic cells,myeloid dendritic cells and plasma dendritic cells were analyzed by using flow cytometer within 24 h.All the data was analyzed with SPSS17.0 software.Results After 3 courses′treatment,the monocytes decreased significantly,from(9.85±2.31)%to(3.67±0.78)%.Dendritic cells increased significantly,from(0.24±0.04)%to(0.58±0.13)%.mDCs increased significantly from(0.11±0.02)%to(0.36±0.08)%,however,pDCs only increased from(0.13±0.03)%to(0.21±0.04)%with no statistically significance.Conclusion After 3 treatment courses with GM-CSF,the monocytes decreased obviously while DCs especially mDCs increased obviously in DLBCL patients.GM-CSF might activate the anti-tumor immunity of DLBCL patients.