6种中草药对多重耐药菌的抗菌活性及逆转耐药性作用的体外研究

Antimicrobial activity and drug-resistant reverse of 6 traditional Chinese medicines against multidrug resistant bacteria in vitro

目的筛选抗菌活性较强并可逆转细菌耐药性的中草药,为治疗多重耐药菌感染提供新的思路。方法以赤芍、青黛、五倍子、野菊花、鱼腥草、小檗碱等6种临床常用抗菌中草药为研究对象,煎煮法制备提取液后,采用2倍稀释法观察6种中草药对多重耐药铜绿假单胞菌(MDR-PA)与多重耐药鲍曼不动杆菌(MDRAB)的抑菌效果;采用纸片扩散(K-B)法检测中草药对多重耐药菌及原始菌株的最低抑菌浓度,计算两者抑菌圈差值,评估中草药逆转多重耐药菌耐药性的效果。结果 6种中草药对MDR-AB与MDR-PA有不同程度的抑制作用,抑菌作用由强到弱依次为五倍子、鱼腥草、野菊花、赤芍、青黛、小檗碱,五倍子对MDR-AB与MDRPA抑菌效价为512~1 024,抑菌作用最强;五倍子与小檗碱对MDR-AB与MDR-PA的敏感性在逆转前后的抑菌圈直径均值比较,差异有统计学意义(P<0.05),但逆转前后抑菌圈直径均值的差值小于3mm。结论赤芍、青黛、五倍子、野菊花、鱼腥草、小檗碱对MDR-AB与MDR-PA有不同程度抑制作用,五倍子抑菌作用最强;五倍子与小檗碱对MDR-AB与MDR-PA的耐药性有一定程度逆转作用。

Objective To filter the traditional Chinese medicine with strong antibacterial activity and effects of reversing drug resistance of bacteria for providing new ideas for the treatment of multi-drug resistant bacteria infection.Methods Six kinds of antibacterial Chinese traditional medicines commonly used in clinic treatment as Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine were used in this study.After preparation of the extract using decocting method,bacteriostatic effects of these six kinds of Chinese medicine on multiple resistant bacteria were detected by double dilution method.To evaluate the effects of Chinese medicines on reversing drug resistance of multi-drug resistant bacteria,minimum inhibitory concentrations for multi-drug resistant bacteria and the original strain were detected by K-B method and calculated the difference of the bacterial inhibition rings.Results The six kinds of traditional Chinese medicines had different degrees of inhibitory effects on MDR-AB and MDR-PA,and the bacteriostatic effect sequence were Galla Chinensis,Herba Houttuyniae,Flos Chrysanthemi Indici,Radix Paeoniae Rubra,Indigo Naturalis,and Berberine.The bacteriostatic titer of Galla Chinensis on MDR-AB and MDR-PA was 512-1 024,whose antibacterial activity was the strongest in the six antibacterial Chinese traditional medicines.Galla chinensis and Berberine had significant effects on reversing drug resistance of MDR-AB MDR-PA(P<0.05),but the difference value of inhibition zone diameters before and after reversing were less than 3 mm.Conclusion Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine could inhibit MDR-AB and MDR-PA in different degrees,in which Galla Chinensis was the most effective antibacterial Chinese Medicine.Ggalla chinensis and Berberine could reverse drug resistance of MDR-AB and MDR-PA to some extent.