熊果酸对大鼠CIA模型关节TNF-α、IL-17、PGE2表达的影响

Effects of Ursolic Acid on the Expression of TNF-α, IL-17 and PGE2 in Joints of CIA Model Rats

目的探讨熊果酸(UA)对胶原诱导性关节炎(CIA)模型大鼠关节中TNF-α、IL-17、PGE2表达的影响。方法首先随机选取8只作为正常对照组,其余复制SD大鼠CIA模型,造模成功后随机分为模型对照组、熊果酸组、甲氨蝶呤组、地塞米松组并给予相应处理。于给药35天后处死大鼠,取关节制作切片,进行HE染色,观察关节骨质的病理改变,免疫组化法检测关节中炎症因子TNF-α、IL-17、PGE2的表达。结果与CIA模型组比较,UA大鼠组关节炎症因子TNF-α、IL-17、PGE2的表达明显降低(P<0.01),UA组效果不如甲氨蝶呤组、地塞米松组(P<0.01)。结论 UA能明显缓解CIA大鼠关节炎症及损伤,减少关节中相关炎症因子的表达。

Objective To investigate the effect of ursolic acid(UA)on the expression of TNF-α,IL-17 and PGE2 in the joints of rats with collagen-induced arthritis(CIA).Methods Eight rats as normal control group were randomly selected from the 60 SD rats,and the other rats were built for CIA models.Then the CIA models were randomly divided into model control group,ursolic acid group,methotrexate group and dexamethasone group.The rats were sacrificed 35 days after the administration,and the sections were harvested for HE staining to observe the pathological changes of the joint bone.The expression of inflammatory cytokines TNF-α,IL-17 and PGE2 were detected by immunohistochemistry.Results Compared with CIA model group,the expression of inflammatory factors TNF-α,IL-17 and PGE2 in UA rats significantly decreased(P<0.01),and the effects of UA were not as good as those in methotrexate and dexamethasone groups(P<0.01).Conclusion UA can significantly relieve the inflammation and injury of joints in CIA rats and reduce the expression of related inflammatory factors in the joints.