HNK盐酸盐药物的血脑屏障通透性试验

Experimental Design of Blood-Brain Barrier Permeability of Hydroxynorketamine Hydrochloride

以HNK盐酸盐药物为试验药品,采用尾静脉注射和灌胃两种给药方式对试验动物直接给药,并在规定的8个时间点收集血液和大脑,将样品进行一系列的处理,在液相质谱仪中进行检测,结果显示:尾静脉给药条件下,血和脑的药物浓度在1 h后达到最低检测限,血脑屏障通透率为1.61;灌胃给药条件下血的药物溶解度在4 h后达到最低检测限,脑的药物溶解度在2 h后达到最低检测限,血脑屏障通透率为0.53。结论显示,这种HNK盐酸盐药物可以透过血脑屏障进入大脑并作用于大脑,且尾静脉给药比灌胃给药的效果更快更好。

Tail intravenous injection and intragastric administration of drug were conducted on ICR mice;after that blood and brain samples were collected at the specified eight time points.Samples were detected by liquid chromatography-mass spectrometry after a series of processing.The results showed that in the situation of intravenous injection,the concentration of HNK hydrochloride drug in blood and brain was lower than minimum detection limit after one hour and the brain permeability ratio was 1.61.In the situation of intragastric administration,the concentration of drug in blood was lower than minimum detection limit after four hours and the concentration of drug in brain was lower than minimum detection limit after two hours and the brain permeability ratio was 0.53.The HNK hydrochloride drug could pass through blood brain barrier and the effect of drug by intravenous injection was better than that by intragastric administration.