10-23型脱氧核酶对HBV S、C基因体外切割作用研究

Cleavage effect of type 10-23 deoxyribozyme on HBV S and C gene in vitro

目的:探讨10-23脱氧核酶(10-23 DRz)对HBV S基因及C基因体外转录产物的特异性切割作用。方法:分别构建含有HBV S基因或C基因的重组质粒,以线性化的重组质粒为模板,体外转录获得相应HBV S基因RNA及C基因RNA。设计并合成针对HBV S基因的DRz-hbvs-1和针对C基因的DRz-hbvc-1,观察其对靶RNA的体外切割作用。结果:DRz-hbvs-1及DRz-hbvc-1能对各自相应的靶RNA进行有效的特异性切割;无DRz对照组及反义寡核苷酸对照组均未见特异性切割。结论:10-23 DRz能特异性切割HBV S基因及C基因体外转录产物。

Objective:To explore the specific cleavage activities of type 10-23 deoxyribozyme 10-23 DRz enzymes on HBV S and C RNA in vitro.Methods:The recombinant plasmids containing HBV S and C gene were constructed,respectively.The HBV S or C gene RNA was synthesized in vitro using the linearized recombinant plasmid as template.The DRz-hbvs-1 and DRz-hbvc-1 targeting to cleave HBV S and C mRNA were designed and synthesized,respectively,and the cleavage activities of which on targeting RNA were observed in vitro.Results:The DRz-hbvs-1 and DRz-hbvc-1 could specifically and effectively cleave the targeting RNA,and the cleavage of which in nonDRz and ASODN control groups were not found.Conclusions:The 10-23 DRz can specifically cleave the HBV S and C gene RNA in vitro.