响应面法优化高效液相色谱-柱前衍生化检测发酵乳制品中的组胺

Optimization of Sample Preparation by Response Surface Methodology for the Determination of Histamine in Fermented Dairy Products by High Performance Liquid Chromatography with Precolumn Derivatization

采用高效液相色谱-柱前衍生化方法,对市售发酵乳制品(天然干酪、再制干酪和发酵乳等)中的组胺含量进行检测。对影响测定结果的衍生试剂(丹磺酰氯)用量、反应p H值、衍生反应时间和衍生反应温度4个因素进行单因素试验和响应面优化多因素交互作用分析。结果表明:优化所得衍生化条件操作简便、高效、测定准确度和精密度均较高,能够满足精确测定乳制品中组胺含量的要求。

The histamine content in commercial fermented dairy products was determined by high performance liquid chromatography(HPLC)with precolumn derivatization.Four derivatization conditions,dosage of derivatizing agent(dansyl chloride,Dns-Cl),pH value,time and temperature were optimized using one-factor-at-a-time method and response surface methodology(RSM).The results showed that the optimized derivatization conditions were easy to manipulate and allowed for efficient,accurate and precise determination of histamine.The method met the requirements for accurate determination of histamine dairy products.