单细胞测序技术及其在传染病研究领域中的应用

Single-cell sequencing technology and its application in infectious disease

单细胞测序技术是在单个细胞水平上对基因组或者转录组进行测序,从而分析相同表型细胞的遗传异质性,或获得难以培养微生物的遗传信息。单细胞测序技术的应用已经深刻地改变了我们对一系列生物学现象的理解,包括基因转录、胚胎发育、癌变。对单细胞测序技术的方法和应用进行了概述,并对其在传染病研究中的应用进行了详细介绍。

Single-cell sequencing technology is sequencing the genome or transcriptome at single cell level,to analyze the genetic heterogeneity of the same phenotype cells,and also to get the genetic information from uncultivated microorganisms.The application of single-cell sequencing technologies has profoundly changed our understanding of a series of biological phenomena,including gene transcription,embryo development and carcinogenesis.Here,we have summarized the methods and application of single-cell sequencing technology,especially its application in infectious disease.