高效液相色谱法同时测定竹叶椒中4种成分含量

Simultaneous Quantification of Four Constituents in Zanthoxylum armatum Using HPLC

建立高效液相色谱法同时测定竹叶椒中桉脂素、N-甲基脱水四氢小檗碱A、芝麻素和planispine A4种成分的含量。采用Diamonsil C18柱(4.6 mm×250 mm,5μm),以乙腈(A)-水(B)为流动相梯度洗脱,流速1 m L/min;柱温30℃;检测波长236 nm。桉脂素在6.25~100μg·m L^(-1)(r=0.9996)范围内、N-甲基脱水四氢小檗碱A在6.25~100μg·m L^(-1)(r=0.9995)范围内、芝麻素在11.25~180μg·m L^(-1)(r=0.9995)范围内、planispine A在9.38~150μg·m L^(-1)(r=0.9998)范围内均线性良好。平均回收率分别为99.31%(RSD=1.47%)、101.34%(RSD=1.39%)、98.03%(RSD=1.97%)、100.39%(RSD=2.10%)。本方法简便、可靠、准确度高、重复性好,可用于竹叶椒多成分质量控制研究。

A high performance liquid chromatography(HPLC)method was developed and validated for simultaneous quantification of four constituents including eudesmin,N-Methylanhydrotetrahydroberberrubine A,sesamin and planispine A in Zanthoxylum armatum.The quantification was performed on Diamonsil C 18 column(4.6 mm×250 mm,5μm),The mobile phase was acetonitrile(A)-water(B)with a gradient elution,at the flow rate of 1 mL/min.The column temperature was 30℃with the detection wavelength of 236 nm.The linear range of eudesmin,N-Methylanhydrotetrahydroberberrubine A,fargesin and planispine A were 6.25~100μg·mL-1(r=0.9996),6.25~100μg·mL-1(r=0.9995),11.25~180μg·mL-1(r=0.9995)and 9.38~150μg·mL-1(r=0.9998),respectively.The average recoveries were 99.31%(RSD=1.47%),101.34%(RSD=1.39%),98.03%(RSD=1.97%),100.39%(RSD=2.10%),respectively.This method was simple,reliable,accurate and reproducible for the quality control of Zanthoxylum armatum.