EGFL-7介导的促血管生成作用在婴幼儿血管瘤中的作用及干预分析

Role of EGFL-7 mediated promoting angiogenesis effect in infantile hemangioma and intervention analysis

目的探讨表皮生长因子样结构域7(EGFL-7)介导的促血管生成作用在婴幼儿血管瘤中的作用及干预措施。方法收集该院2013年2月至2016年3月婴幼儿血管瘤的50例石蜡组织标本,按照病理期的不同分为增生期组、消退期组、消退完成期组。采用自身对照设计,选取5例癌旁正常组织作为对照组。对组织标本进行苏木素-伊红(HE)染色,检测以上病理标本中EGFL-7的生成情况。选取同期收治的30例增生期血管瘤患儿为研究对象,采用普萘洛尔干预治疗,检测该组患儿治疗前后血清、尿液中EGFL-7表达的变化。结果增生期血管瘤组织切片管腔不明显,血管结构紊乱,细胞核大,染色深,内皮细胞排列紊乱。EGFL-7在增生期与消退期组的表达高于消退完成期组和对照组,差异有统计学意义(P<0.05)。EGFL-7在消退完成期组与对照组的表达差异无统计学意义(P>0.05)。血管瘤的患儿经普萘洛尔干预后血清、尿液中的EGFL-7均低于干预前,差异有统计学意义(P<0.05)。结论经普萘洛尔干预后EGFL-7水平显著下降,这可能成为血管瘤干预的重要机制。

Objective To explore the role of EGFL-7 mediated promoting angiogenesis effect in infantile hemangioma and intervention measures.Methods Fifty paraffin tissue specimens from infantile hemangioma in this hospital from February 2013 to March 2016 were collected and divided into the hyperplasia stage group,fading stage group and fading complete stage group according to the different pathological stages.The tissue samples conducted HE staining.The EGFL-7 generation situation in the pathological sample was detected.Thirty cases of hyperplasia stage hemangioma treated at the same period were selected as the research subjects.The propranolol intervention treatment was adopted.The EGFL-7 expression changes in serum and urine were detected before and after treatment.Results In the tissue section of hyperplasia stage hemangioma,the lumen was not obvious,the vascular structure was disorder with large nucleus,deep staining,disordered arrangement of endothelial cells.The expression of EGFL-7 in the hyperplasia stage and fading stage was higher than that in the fading complete stage group and normal skin,the difference was statistically significant(P<0.05).The EGFL-7 expression difference between the fading complete stage and normal skin had no statistical significance(P>0.05).Serum and urine EGFL level after propranolol intervention in hemangioma infants was lower than that before treatment,the difference was statistically significant(P<0.05).Conclusion The EGFL-7 level is significantly decreased after propranolol intervention,which may become the important mechanism of hemangioma intervention.