新霉素分批发酵过程特性与动力学研究

Studies on the Characteristics of Neomycin Batch Fermentation Process and Kinetics

对弗氏链霉菌(Streptomyces fradiae)产新霉素的发酵过程研究表明菌体生长和产物合成过程具有典型的非生长耦联特征,发酵过程最大比生长速率为0.0612h-1,产物最大比合成速率为3.2×10-4g·g-1·h-1。建立了新霉素分批发酵过程产物合成期的动力学模型,对模型参数进行了估计和回归,模型拟合平均偏差小于8.5%,可用于新霉素发酵过程优化控制。

The research on the fermentation process of Streptomyces fradiae producing neomycin shows that cell growth and product synthesis process take on a typical non-growth coupled characteristics.The maximum specific growth rate of the fermentation process was 0.0612 h-1 and the synthetic rate of the product maximum ratio was 3.2×10-4g·g-1·h-1.The kinetic model for product synthesis period of a batch fermentation process was established and the model parameters were estimated and regressed.The average deviation of model fitting is less than 8.5%which can be used to optimize the control of neomycin fermentation process.