番茄生防内生细菌的分离鉴定及耐高温高盐特性研究

Isolation and Identification of Endophytic Bacteria in Tomato and Its Tolerance on High Temperature and High Salinity

为筛选具有良好生防效果和生产应用价值的番茄内生菌菌株,从番茄植株内部分离得到一株对番茄灰霉病病原菌具有较高拮抗效果的内生细菌JZ3。通过菌株形态学特征比较、16S r DNA序列和gyr B基因序列系统进化分析,结果显示JZ3为解淀粉芽孢杆菌。室内生测结果表明,浓度为5×107cfu/m L的JZ3液体悬浮剂对番茄灰霉病的预防效果可达82.04%,治疗效果约为30.54%。检测JZ3对高温高盐耐受性,结果 JZ3在高盐环境中的存活率可达70%,105℃高温高盐条件下处理15 min存活率仍可达56%左右。检测JZ3的储藏稳定性,结果显示,室温环境下储存12个月,有效活菌数基本保持不变。综上所述,番茄内生解淀粉芽孢杆菌JZ3具有良好的生防和加工储藏性能,为开发高效的微生物制剂提供了良好的菌种资源,具有广阔的应用前景。

In order to screen tomato endophytic bacteria strains with good biological control effect and application value.In this study,an endophytic bacterium named JZ3 with high antagonistic activity against tomato grey mold was isolated.Through the comparison of morphological characteristics,phylogenetic analysis of 16S rDNA sequence and gyrB gene sequence,the results showed that JZ3 was a strain of Bacillus amyloliquefaciens.The results of laboratory test showed that,the preventive effect of JZ3 liquid suspension(5×10 7 cfu/mL)on the tomato gray mold was up to 82.04%,the treatment effect was about 30.54%.The high temperature and high salinity tolerance of JZ3 was detected,the results showed that the survival rate of JZ3 in high salinity environment was 70%,the survival rate of JZ3 under high temperature and high salt condition(15 min)was still up to 50%.The storage stability of JZ3 was tested,the results showed that number of functional microorganism remained unchanged after storaged in the room temperature for 12 months.In conclusion,the Bacillus amyloliquefaciens strain JZ3 isolated from tomato plants has good biocontrol and storage properties,it provides a good strain resource for the development of efficient microbial preparation,and has broad application prospects.