岩生树花地衣型真菌NRPS基因的生物信息学分析

The Aioinformatics of NRPS Gene in Ramalina intermedia

为了挖掘岩生树花地衣型真菌转录组数据中的NRPS基因,找出其潜在化合物或蛋白,以岩生树花地衣型真菌的转录组数据为基础,分析获得一个新的NRPS基因(Ri NRPS)。利用BLAST比对、分子系统进化及anti SMASH、NRPS predictor、Na PDo S、Norine等生物信息学分析手段对岩生树花地衣型真菌Ri NRPS基因进行分析,结果显示,Ri NRPS基因相对分子质量为702 699.9,等电点5.83,为不稳定蛋白;其开放阅读框总长19 098bp,编码6 362个氨基酸;该蛋白不存在信号肽,为非分泌蛋白,定位于细胞质基质中;可能合成HC-toxin合成酶,可能的催化反应是以Orn(鸟氨酸)、Gln(谷氨酰胺)、Gln(谷氨酰胺)、Cys(半胱氨酸)4个氨基酸作为底物合成环状四肽,此环状四肽可能是一种具毒素活性的多肽、其结构式为C21H32N4O6。研究结果为以后岩生树花NRPS基因的挖掘及非核糖体多肽类物质的生物合成提供参考。

In order to searching the NRPS gene,the putative compound or protein of Ramalina intermedia lichen forming fungi,we obtained a novel NRPS gene(RiNRPS)by analysed the transcriptom of R.intermedia lichen forming fungi.We checked the RiNRPS gene using the BLAST,phylogenetic tree analysis and antiSMASH,NRPS predictor,NaPDoS,Norine bioinformatics,the results showed that:the relative molecular weight of the RiNRPS gene is 702 699.9,pI is 5.83,and unstable protein;the full length of RiNRPS open reading framer takes 19 098bp,and codes 6 362 amino acids,non-exist signal peptide,is an unsecreted protein,locates in the cytoplasmic matrix;it might code HC-toxin synthetase.The HC-toxin synthetase could catalyze Orn,Gln,Gln and Cys into cyclic tetrapeptides,the compound may be an polypeptides having the bioactivity of toxin,and the constitutional formula is C 21 H 32 N 4 O 6.The study should apply some basis of gene mining of NRPS gene from Ramalina intermedia and the biosynthesis of nonribosomal peptides.