适配体光子晶体传感材料的制备及初步应用

Preparation and Application of Aptamer Photonic Crystal Sensing Materials

目的制备高度有序稳定的反蛋白石光子晶体材料,并将其应用于黄曲霉毒素B1的快速检测。方法以聚甲基丙烯酸甲酯(PMMA)微球为模板,基于溶液中胶体/模板共组装法制备SiO_2反蛋白石光子晶体材料。以黄曲霉毒素B1(AFB1)为靶标,以适配体作为识别元件,制备适配体光子晶体传感材料,采用间接竞争法,建立黄曲霉毒素B1的快速检测方法。结果该方法可控合成大面积高度有序的三维结构材料,并进一步制备了适配体光子晶体传感材料,初步实现了黄曲霉毒素B1的快速检测,最低检测限达10^(-3)ng/ml。结论该方法可防止硅溶胶过度覆盖和非均匀渗透,减少了随机缺陷,简化了制备步骤。此外,还可修饰某些官能团制备成传感检测材料,如修饰氨基后,可通过戊二醛连接适配体、蛋白、抗体以及受体等,极大地拓展了光子晶体的应用领域。

Objective To prepare highly ordered and stable inverse opal photonic crystal materials and apply them to rapid detection of aflatoxin B1.M ethods The SiO2 inverse opal photonic crystal(PC)materials were prepared through co-assembly of poly(m ethyl methacrylate)(PMMA)microspheres and TEOS solution.Subsequently,a rapid detection method of aflatoxin B1(AFB1)was preliminarily established using this PC based on indirect competition with the aptamer as the recognition element.Results This method could obtain a large area of materials with a highly ordered 3-D structure.Then a rapid detection method of aflatoxin B1 was achieved based on such materials.The limit of detection(LOD)was 10-3ng/ml.Conclusion This method can prevent the overlayer and nonuniform infiltration of TEOS solution.Furthermore,this method can reduce random defects and simplify the steps of preparation.In addition,some functional groups can be modified on PC to prepare the sensing materials.For example,after modification of amino,the aptamer,protein,antibody,and receptor can be attached to PC by glutaraldehyde,which greatly improves the applicability of PC.