黑龙江省马铃薯晚疫病菌的交配型和multi-locus基因型分析

Analysis of Mating Type and Multi-locus Genotypes of Phytophthora infestans Collected from Heilongjiang Province

2015~2017年在黑龙江省哈尔滨市、绥化市和齐齐哈尔市各马铃薯主产区共采集分离了126株马铃薯晚疫病菌菌株,测定交配型、mtDNA单倍型、SSR基因型并进行multi-locus基因型分析。结果显示,126株菌株中发现了A1、A2、自育型3种交配型,分别占分离菌株总数的88.1%、6.3%和5.6%。126株菌株中共鉴定出Ⅰa和Ⅱa两种mt DNA单倍型,分别占分离菌株总数的17.5%和82.5%。126株菌株中共鉴定出7种SSR基因型,分别为F-01、F-02、F-03、F-05、F-06、D-03和G-02,其中F-01基因型(77.8%)为优势基因型。根据马铃薯晚疫病菌的mt DNA单倍型和SSR基因型,共划分了9种multi-locus基因型,其中multi-locus基因型A(65.1%)是黑龙江省发现的优势基因型。2015~2017年黑龙江省马铃薯晚疫病菌群体结构逐年复杂,绥化市马铃薯晚疫病菌群体结构最为复杂。

A total of 126 Phytophthora infestans isolates were collected from Harbin,Suihua and Qiqihar Cities of Heilongjiang Province from 2015 to 2017.Analysis on their mating types,mtDNA haplotypes,SSR genotypes and multi-locus genotypes were carried out.The results indicated that 3 mating types of A1,A2 and self-fertility were found in 126 strains isolated,accounting for 88.1%,6.3%and 5.6%of the total isolates,respectively.Two mtDNA haplotypes,Ⅰa andⅡa,were identified,accounting for 17.5%and 82.5%of the total number of isolates,respectively.Seven SSR genotypes,F-01,F-02,F-03,F-05,F-06,D-03 and G-02,were identified from 126 isolates tested.Among them,the F-01 genotype(77.8%)was dominant genotype.A total of 9 multi-locus genotypes were identified by systematic analysis of P.infestans,and the multi-locus genotype A(65.1%)was the dominant genotype found in Heilongjiang Province.These results indicated that from 2015 to 2017,the population structure of P.infestans in Heilongjiang Province was very complicated year by year.And the population structure of P.infestans in the Suihua City was the most complex.