Nd:YAG激光对具核梭杆菌的杀灭作用

Antibacterial effect of Nd: YAG laser on Fusobacterium nucleatum

目的·观察Nd:YAG激光对具核梭杆菌(Fusobacterium nucleatum,F.nucleatum)的杀灭效果。方法·使用结晶紫染色,观察Nd:YAG激光对浮游状态F.nucleatum成膜能力的影响;运用激光共聚焦显微镜扫描分析,观察激光对单菌种生物膜中F.nucleatum活力的影响;使用扫描电子显微镜技术,观察激光对单菌种生物膜中F.nucleatum结构的破坏。结果·激光照射15s对浮游状态下F.nucleatum成膜能力有抑制作用,激光照射35s对生物膜状态下F.nucleatum活力产生抑制。激光能破坏生物膜及生物膜中细菌的结构,且随光照时间延长,破坏程度增加。结论·Nd:YAG激光能够抑制浮游状态F.nucleatum的成膜能力,对单一菌种生物膜中细菌活力和细菌结构产生破坏,从而达到杀菌效果。

Objective·To investigate the antibacterial effect of Nd:YAG laser on Fusobacterium nucleatum(F.nucleatum)in vitro.Methods·The laser effect on the biofilm formation ability of planktonic F.nucleatum were observed by crystal violet test;confocal laser scanning microscopy(CLSM)was used to detect the effect on the bacteria viability of mature biofilm;scanning electron microscopy(SEM)was performed to investigate the effect on the morphology of mature bioflm.Results·After 15 s radiation the biofilm formation ability of F.nucleatum went down.The viability of F.nucleatum in mature biofilm went down under 35 s iradiation.The deformation of mature biofilm and bacterium became distincted with the iradiation time raised.Conclusion·Nd:YAG laser shows the inhibition of biofilm formation ability on planktonic F.nucleatum.The vitality of F.nucleatum in mature biofilm is depressed after laser radiation.Laser can destroy the construction of the mature biofilm and bacteria.