2014-2016年四川省疟疾诊断参比实验室结果比较

Comparison of malaria diagnosis in Sichuan province from 2014 to 2016

目的:以四川省省级疟疾诊断参比实验室复核结果为对照,评价县级疟疾实验室诊断能力,分析存在的问题。方法:选取2014年1月至2016年12月四川省各县级疫情报送单位中疑似、临床、确诊为疟疾病例的血片及血样945例。省级疟疾诊断参比实验室分别采用镜检以及巢氏PCR方法对样本进行复核,为县级实验室疟疾诊断能力评价,及现存问题分析提供依据。结果:945例样本中,以省级PCR和镜检检测的一致结果为依据进行统计,县级镜检的定性阳性符合率为86.75%;分型阳性符合率为79.11%,其中间日疟86.96%,恶性疟84.58%,卵形疟20.00%,三日疟80.00%,混合感染为50.00%。结论:我省需进一步针对性强化培训,加强疟疾诊断意识,提高县级疟原虫实验室诊断能力。

Objective:To evaluate the diagnostic ability of malaria laboratory in county level in Sichuan province,and to analyze the existing problems.The consistency between PCR and microscopic results in the provincial laboratory was set as the basis for evaluation of microscopic results in county laboratories.Methods:Nine hundred and forty-five blood tablets and blood samples of suspected,clinical,confirmed malaria cases from the county CDC were selected from January 2014 to December 2016 in Sichuan Province.The samples were re-examined by microscopy and nested-PCR respectively in the provincial malaria diagnostic reference laboratory.Results:Of all the 945 samples,based on the consistent results of provincial PCR and microscopic examination,the coincidence rate of county level microscopy was 86.75%;the type of positive rate was 79.11%,86.96%of PV,84.58%of PF,20.00%of PO,80.00%of PM,and mixed infection was 50.00%.Conclusion:It is important to strengthen training,the awareness of malaria diagnosis and improve laboratory diagnostic level.