利用中效原理评价盐霉素与三氧化二砷联合对乳腺癌MCF-7细胞的抑制作用

Evaluation of the inhibitive effects of salinomycin and arsenic trioxide on breast cancer cell MCF-7 by using median-effect principle

目的研究盐霉素(salinomycin,Sal)与三氧化二砷(arsenic trioxide,As_2O_3)联合应用对体外人乳腺癌MCF-7细胞的生长抑制作用。方法采用MTT法检测单药及联合用药对MCF-7细胞株的抑制效应;利用中效原理(median-effect principle)评价2种药的相互作用;通过细胞集落实验和划痕试验观察2种药物对细胞增殖和迁移的影响;利用Hoechst33258染色法观察单药及联合用药对MCF-7细胞凋亡的形态变化。结果 2种药物单用及合用时随药物剂量的增加,其效应(fa)也随之增加,当fa<0.827 55时,合用指数<1,2种药合用效用表现为协同作用。结论低剂量的Sal和As_2O_3联合应用能协同性地抑制MCF-7细胞增长和促进其凋亡。

To study the inhibitive effects of salinomycin in combination with arsenic trioxide(As 2O 3)on the growth of vitro human breast cancer cell line MCF-7.The inhibitive effects of single drug and combined medication on the cell line MCF-7 were detected by using MTT method.The interactions of the 2 drugs were assessed by using median-effect principle,and the effects of the 2 drugs on cell proliferation and migration were observed by colony and scarification tests,and the effects of single drug medication and combined drug medication on the morphology of MCF-7 apoptosis were observed by Hoechst 33258 staining.The effects of single drug medication and combined drug medication increased with the dosage of the drugs.When fa was lower than 0.827 55 and CI was lower than 1,the combined effects of the 2 drugs were synergistic.Combined medication of the 2 drugs at low dosage could synergistically inhibit the growth of MCF-7 and could further promote apoptosis.