紫背天葵MBW相关调控因子转录组测序分析

Sequence Analysis of MBW Related Genes Transcriptome in Gynura bicolor

为获取紫背天葵(Cynura bicolor)花青素合成相关转录调控因子MBW家族基因,采用二代高通量测序技术进行全转录功能基因组测序后组装,再通过Pfam、Swiss Prot和Nr数据库搜索,共获得138个MBW相关Unigene,分别有42个MYB、67个b HLH、15个b HLH-MYB和14个WD40,其中目前已报道与花青素合成代谢相关的MYB、b HLH、b HLH-MYB和WD40分别为11、33、6和3个。这为进一步研究紫背天葵花青素合成的调控机理和相关基因克隆等奠定基础。

Gynura bicolor is rich in anthocyanidin.In order to identify the transcription factors involved in anthocynidin biosynthesis pathway in G.bicolor,the transcriptome of this plant was sequenced by Illumina HiSeq 2500 platform.By searching of 3 databases,including Pfam,SwissProt and Nr,138 MBW-related unigenes were obtained,which containing 42 MYBs,67 bHLHs,15 bHLH-MYBs and 14 WD40s.Among these MBWs,11 MYBs,33 bHLHs,6 bHLH-MYBs and 3 WD40s involved in anthocynidin biosynthesis pathway were annotated in other plant species,including Vitis vinifera,Dahlia pinnata,Gerbera,Catharanthus roseus,Prunus persica etc.The obtaining of the 138 unigenes related to MBWs from Gynura bicolor provides a good basis for further research in gene cloning and the regulation of anthocyanin synthesis.