桂花叶的化学成分及抗肿瘤活性研究

Study on Chemical Constituents and Antitumor Activities of Leaves of Osmanthus fragrans

为了解桂花(Osmanthus fragrans)叶片的化学成分,应用多种色谱分离手段从桂花叶醇提溶液分离得到13个化合物,经波谱分析分别鉴定为香橙素(1)、染料木黄酮(2)、lupinalbin A(3)、齐墩果酸甲酯(4)、3-羰基齐墩果酸(5)、fouquierol(6)、19α-羟基-3-O-乙酰熊果酸(7)、白桦脂醇(8)、豆甾-3,5-二烯-7-酮(9)、刺五加酮(10)、对羟基苯乙酮(11)、3,16α-二羟基-对映贝壳杉烷(12)和trigoflavidone A(13)。所有化合物均为首次从桂花中分离得到。采用MTT法检测抗肿瘤活性表明,化合物2、3、10均有一定的抗肿瘤活性,且对4种肿瘤细胞株的活性检测为首次报道。

In order to understand the chemical constituents of Osmanthus fragrans leaves,thirteen compounds were isolated from the 95%ethanol extract.On the basis of spectral data,their structures were identified as aromadendrin(1),genistein(2),lupinalbin A(3),methy oleanolate(4),3-ketooleanolic acid(5),fouquierol(6),19α-hydroxyl acetylursolic acid(7),betulin(8),stigmasta-3,5-dien-7-one(9),ciwujiatone(10),p-hydroxyacetophenone(11),entkaurane-3,16α-diol(12),and trigoflavidone A(13).All of compounds are isolated from the leaves of O.fragrans for the first time.The compounds 2,3,10 showed significant antitumor activities,and their activity determination to 4 kinds of tumor cells was reported for the first time.