玉米大斑病广谱抗性外引自交系的发掘与抗病基因初步鉴定

Exploration of Foreign Maize Inbred Lines with Broad Spectrum Resistance to Northern Corn Leaf Blight and Preliminary Identification of Resistance Genes

从2014—2016年连续3年对43份来自美国、法国、俄罗斯和德国的玉米资源进行了抗大斑病人工接种鉴定,筛选到高抗玉米大斑病材料7份,抗病材料1份,中抗材料6份,抗性材料占鉴定总材料的比例为32.6%。利用F_2群体,对7份高抗材料进行了大斑病抗性遗传分析,抗感植株分离比例和适合性测验证明,自交系A04、F02、F05和R01对大斑病的抗性可能是由一对显性基因控制的。抗谱分析表明,自交系A04、F02、F05和R01携带的抗大斑病基因不同于Ht1、Ht2、Ht3和Ht N,可能是新的抗病基因。该研究结果可为今后我国玉米大斑病抗性种质的引进及改良提供重要参考。

From 2014 to 2016,forty-three maize inbred lines introduced from the United States,France,Russia and Germany,were identified and evaluated for resistance to northern corn leaf blight(NCLB).The results showed that seven,one and six maize inbreds were highly resistant,resistant or moderately resistant to NCLB,accounting for 32.6%.Exserohilum turcicum was used to test the F2 populations for analyzing the inheritance of NCLB resistance in the seven highly resistance maize inbred lines.Genetic analysis suggested that A04,F02,F05,and R01 of these inbred lines probably carried a single dominant gene conferring their resistance to NCLB.The reaction patterns to isolates 0,1,2,N,and 123N of E.turcicum showed that new genes resistance to NCLB were most likely present in A04,F02,F05,and R01.The results provided useful information on the introduction of maize germplasms and genetic improvement for resistance to NCLB