TIPE2在溃疡性结肠炎中的表达及其意义探究

Expression and Significance of TIPE2 in Ulcerative Colitis

背景:TIPE2是一种新近发现的免疫负调控蛋白,对于维持免疫稳态和免疫耐受具有重要意义。研究发现TIPE2在人体中广泛表达于各类组织和器官。目的:检测TIPE2在溃疡性结肠炎(UC)患者和非UC个体外周血和结肠黏膜中的表达,探讨其在UC发生、发展中的作用。方法:收集2015年1月—2016年8月就诊于西安交通大学第二附属医院的活动期UC患者的外周血标本42例和结肠黏膜标本30例,同时采集同期非UC个体外周血和结肠黏膜标本作为对照,分别采用real-time PCR和免疫组化染色检测外周血单个核细胞TIPE2 mRNA和结肠黏膜TIPE2蛋白表达。结果:TIPE2 mRNA在UC患者外周血中的表达较对照组有增高趋势,但差异无统计学意义(P>0.05);Truelove-Witts分型轻、中、重度UC组间TIPE2 mRNA表达总体差异无统计学意义(P>0.05)。TIPE2蛋白在UC患者结肠黏膜中的表达明显高于对照组,差异有统计学意义(P<0.05);其表达随UC组织学分级的升高而升高,但总体差异无统计学意义(P>0.05)。结论:TIPE2在UC患者结肠局部表达增高,可能与UC发生、发展相关。

Background:TIPE2 is a newly identified negative regulator of innate and adaptive immunity that maintains immune homeostasis and immune tolerance.It has been demonstrated that TIPE2 is expressed in a wide variety of tissues and organs in humans.Aims:To investigate the expression of TIPE2 in peripheral blood and colonic mucosa of patients with ulcerative colitis(UC)and non-UC subjects,and to explore the role of TIPE2 in the initiation and development of UC.Methods:Forty-two peripheral blood samples and 30 colonic mucosa samples from patients with active UC were collected during Jan.2015 to Aug.2016 at the Second Affiliated Hospital of Xian Jiaotong University.Peripheral blood and colonic mucosa samples from non-UC subjects were served as controls.Real-time PCR and immunohistochemical staining were used to detect the mRNA and protein expressions of TIPE2 in peripheral blood mononuclear cells and colonic mucosal tissues,respectively.Results:Expression level of TIPE2 mRNA in peripheral blood of UC patients had a trend to increase but no significant difference was found between UC patients and the controls(P>0.05).When classified by Truelove-Witts severity index,there was no significant difference among patients with severe,moderate and mild UC(P>0.05).Expression level of TIPE2 protein was significantly higher in colonic mucosa of UC patients than that of the controls(P<0.05);the expression level increased with increase of histological grade of UC,but the difference was not statistically significant(P>0.05).Conclusions:Increased colonic expression of TIPE2 might contribute to the initiation and development of UC.