芦荟凝胶对成纤维细胞紫外辐射损伤的保护作用

Protective effect of Aloe vera gel against UV-induced damages of fibroblast

为了探讨芦荟凝胶对紫外辐射所致皮肤成纤维细胞氧化损伤的保护作用和机制,利用长波紫外线(UVA)辐射体外培养小鼠成纤维细胞株(NIH 3T3),建立紫外线损伤成纤维细胞模型。UVA辐射NIH 3T3细胞后,分别采用CCK-8法以及生化比色法检测不同质量浓度的芦荟凝胶对其增殖及抗氧化酶活性的影响。结果显示,UVA辐射对NIH 3T3细胞造成明显损伤,100、200、400μg·m L^(-1)芦荟凝胶均可以提高受UVA辐射损伤的NIH 3T3细胞的增殖活性,提高细胞中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的含量,降低脂质过氧化物酶的活性,且呈剂量依赖性。说明UVA对NIH 3T3细胞有损伤作用,100~400μg·m L^(-1)的芦荟凝胶可以拮抗UVA所致的NIH 3T3细胞损伤,具有光保护作用。

The long wave ultraviolet(UVA)was used to radiate mouse fibroblast cell line(NIH 3T3)and establish a model of cell injury in order to probe the protective function and mechanism of Aloe vera gel in cell oxidative damage.After UVA radiated NIH 3T3 cells,the effects of different concentrations of Aloe vera gel on the proliferation and antioxidant enzyme activities of NIH 3T3 cells were detected by CCK-8 and biochemical colorimetric assay respectively.The results show that UVA radiation caused a significant damage to the NIH 3T3 cell,but 100,200,400μg·mL-1 Aloe vera gel can improve the proliferation activity of damaged cells,increase the content of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in cells.It can simultaneously decrease the activity of lipid peroxidase.In conclusion,that UVA could damage NIH 3T3 cells,while 100~400μg·mL-1 Aloe vera gel can antagonize the damage of NIH 3T3 cells induced by UVA and have the effect of light protection.