海洋酸化对青蛤免疫指标的影响

Effect of ocean acidification on the immune index of Cyclina sinensis

通过向水体中通入CO_2的方法模拟海洋酸化环境,测定青蛤(Cyclina sinensis)在酸化条件下各免疫指标的变化情况。结果显示:将青蛤置于酸化的海中(pH分别为7.4和7.7),并以自然海水做对照组(pH 8.1)后,血细胞总数随海水酸化胁迫时间的延长,表现为下降趋势,且差异显著(P<0.05);海水pH降低抑制了溶菌酶的活性,但差异性不显著(P>0.05);ACP活性总体呈下降趋势,对照组活性要高于酸化组,而ALP活性表现为上升趋势。酸化胁迫初期诱导SOD活性升高,后期SOD活性受到抑制,而CAT变化却截然相反;脂质过氧化产物MDA在酸化后期出现显著降低(P<0.05)。

To measure the immune index of Cyclina sinensis under acidification environments,CO2 was dissolved into the seawater.It was found that the total blood cells count had a significantly decreasing trend with increasing acidification time(P<0.05);decrease in pH levels inhibited the activity of lysozyme,but the difference was not statistically significant(P>0.05).The ACP activity was generally deceased,but higher in the control group than the acidification group.However,the ALP activity showed an upward trend.On the other hand,the activity of SOD increased during the initial stages of acidification stress but was inhibited in the later stages.However,the opposite trend was observed for CAT activity;lipid peroxidation products-MDA decreased significantly in the later stage of acidification(P<0.05).