摘要
目的:探讨肝癌细胞发生内质网应激时,细胞中趋化因子的表达情况。方法:培养HepG2细胞,用荧光定量PCR检测经不同浓度衣霉素(TM)刺激后,趋化因子CXCL1、CXCL2、CXCL3、CXCL8的mRNA水平,用ELISA方法检测培养基中的CXCL1含量。结果:TM刺激Hep G2细胞后,CXCL1、CXCL2、CXCL3的mRNA水平显著升高,而CXCL8的mRNA水平降低,其中CXCL1的mRNA水平呈剂量依赖性。结论:内质网应激影响细胞内趋化因子的表达,不同因子的表达趋势有差别。
Objective:To investigate the expression of chemokines in cells when endoplasmic reticulum stress occurs in hepatocellular carcinoma cells.Methods:The HepG2 cells were cultured.The mRNA levels of chemokines CXCL1,CXCL2,CXCL3 and CXCL8 were detected by real-time quantitative PCR after stimulated with tunicamycin(TM).The content of CXCL1 in culture medium was detected by ELISA.Results:After stimulating HepG2 cells with TM,the mRNA levels of CXCL1,CXCL2 and CXCL3 increased significantly,while the mRNA level of CXCL8 decreased.Among them,CXCL1 mRNA level showed a dose-dependent manner.Conclusion:Endoplasmic reticulum stress affects the expression of intracellular chemokines,and the expression trends of different factors are different.
作者
毛瑞涛
陈伟
邹岭
李自慧
叶甲舟
白涛
陈洁
陈健康
王翠
刘宁
杨晓丽
吴飞翔
MAO Rui-Tao;CHEN Wei;ZOU Ling;LI Zi-Hui;YE Jia-Zhou;BAI Tao;CHEN Jie;CHEN Jian-Kang;WANG Cui;LIU Ning;YANG Xiao-Li;WU Fei-Xiang(China-Japan Union Hospital,Jilin University,Changchun 130000,China;Department of Hepatobiliary Surgery,Affili.ated Tumor Hospital of Guangxi Medical University,Nanning 530021,China;Affiliated Hospital of Xiangnan Universi.ty,Chenzhou 423000,China;Clinical Laboratory,General Hospital of Chinese People's Armed Police Forces,Beijing 100039,China)
出处
《生物技术通讯》
CAS
2018年第2期247-251,共5页
Letters in Biotechnology
基金
区域性高发肿瘤早期防治研究教育部重点实验室(GKZ201604)
广西卫计委重点课题(S201513)
关键词
趋化因子
内质网应激
肝细胞癌
chemokine
endoplasmic reticulum stress
hepatocellular carcinoma
