大叶蒲公英愈伤组织耐盐突变体诱导与鉴定

Induction and Identification of Salt-tolerant Mutant of Taraxacum mongolicum Callus

为获得大叶蒲公英耐盐突变体,以其叶片为外植体,进行了愈伤组织耐盐突变体的筛选。结果表明,愈伤组织诱导和增殖的最佳培养基分别为MS+6-BA 0.5 mg/L+2,4-D 0.04 mg/L与MS+6-BA 0.4 mg/L+2,4-D 0.02 mg/L+NAA 0.2 mg/L。将继代培养5次的愈伤组织接种在含有不同质量浓度盐的诱导培养基上,经过4次耐盐胁迫,筛选出了耐盐的愈伤组织突变体。通过RAPD检测发现,在NaCl质量浓度为8、10、12 g/L的培养基上愈伤组织扩增条带与不含NaCl的培养基(对照)有差异,证明耐盐的愈伤组织中确实产生了DNA的突变。

To obtain salt-tolerant mutants of Taraxacum mongolicum,using its leaves as explants the callus of salt-tolerant mutant was screened.The results indicated that the optimal medium for callus induction and proliferation was MS+6-BA 0.5 mg/L+2,4-D 0.04 mg/L and MS+6-BA 0.4 mg/L+2,4-D 0.02 mg/L+NAA 0.2 mg/L respectively.The salt-tolerant mutants were inoculated on medium with different salt concentrations,and screened out from the callus after 4 times of salt stress.Compared with the control without NaCl,there were different bands in the callus cultured on the medium with NaCl of 8,10 and 12 g/L using RAPD detection.It could be coucluded that the DNA of salt tolerant callus mutated.