一株毛色二孢菌发酵产茉莉酸

Production of jasmonic acid by a strain Lasiodiplodia iranensis

从实验室保存和自然界分离到的100多株霉菌中筛选到1株高产茉莉酸的毛色二孢菌Lasiodiplodiairanensis DWH-2。优化得到发酵培养基组分为:蔗糖50 g/L,NaNO_34.5 g/L,KH_2PO_42.0 g/L,KCl 0.4 g/L,MgSO_4·7H_2O 6 g/L,FeSO_4·7H_2O 0.9 g/L,玉米浆1.5 g/L,微量元素10 ml/L,大豆油10 g/L;发酵初始p H 5.5,装液量100 m L/500 m L,培养温度28℃。在此条件下,发酵7 d,茉莉酸产量可达1 543 mg/L。

A strain which could produce a high concentration of jasmonic acid was screened from more than 100 molds in nature and preserved in the laboratory.It was named as Lasiodiplodia iranensis DWH-2.Optimized fermentation medium composition contained sucrose 50 g/L,NaNO3 4.5 g/L,KH 2PO4 2 g/L,KCl 0.4 g/L,MgSO4·7H2O6 g/L,FeSO4·7H2O 0.9 g/L,corn pulp 1.5g/L,trace element 10 ml/L,soybean oil 10g/L,and its initial pH value was 5.5.The culture media volume and culture temperature was optimized to 100 mL/500 mL and 28℃,respectively.Under these conditions,the yield of jasmonic acid could reach 1.543 g/L after 7 days.