超声波辅助提取牡丹籽毛油的工艺优化及脂肪酸组成分析

Optimization of ultrasonic-assisted extraction of peony seed oil and its fatty acid composition

以油用牡丹籽为原料,利用超声波辅助提取牡丹籽毛油,通过单因素及正交试验,研究提取试剂、超声时间、超声温度、超声功率和料液比对牡丹籽毛油提取率的影响,确定超声波辅助提取牡丹籽毛油的最佳工艺条件,并用气相色谱仪分析牡丹籽毛油的脂肪酸组成。结果表明,超声波辅助提取牡丹籽毛油的最佳提取条件为:以正己烷作为提取试剂,超声温度为50℃,超声时间为50 min,超声功率为140 W,料液比为1∶15(g∶m L)。在此条件下,牡丹籽毛油的提取率为(26.11±0.01)%。气相色谱分析显示,牡丹籽毛油的主要成分为棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸,其中不饱和脂肪酸含量高达(92.35±0.51)%,亚油酸和亚麻酸的含量分别为(28.80±0.24)%和(41.13±0.09)%,说明牡丹籽油是一种营养价值很高的食用油脂。

Peony seed were extracted by ultrasonic-assisted extraction to produce peony seed oil.The influence of extraction reagent,time,temperature,ultrasound power and liquid-solid ratio on the peony seed oil extraction rate were studied by single factor and orthogonal test.The optimum process conditions of ultrasonic-assisted extraction of peony seed oil was obtained and the composition of peony seed oil fatty acid was analyzed by Gas chromatography.The results showed that the best extraction conditions of ultrasonic-assisted extraction of peony seed oil were:extraction reagent N-hexnane,extraction time 50min,extraction temperature 50 C,ultrasound power 140W,and liquid-solid ratio 1∶15(g∶mL).Under the above conditions,the peony seed oil extraction yield was(26.11±0.01)%.Gas chromatography analysis showed that the main composition of peony seed oil is palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid.Unsaturated fatty acid content was as high as(92.35±0.51)%,linoleic acid and linolenic acid content were(28.80±0.24)%and(41.13±0.09)%,respectively.Peony seed oil is a high nutritional edible oils.