结直肠癌患者围手术期血清TNF-α、NO、IL-1β、IL-6变化的临床研究

Clinical study on changes of serum TNF-α,NO,IL-1 and IL-6 in patients with colorectal cancer during perioperative period

目的探讨结直肠癌患者围手术期血清肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、白细胞介素(IL)-1β、IL-6水平变化的临床意义。方法选择该院2014年12月至2016年12月收治的行根治性手术结直肠癌患者73例作为观察组。同时选择该院同期健康体检者42例作为对照组。分别于患者入院后第2天(术前)、术后第3天采集患者外周静脉血5mL,分离血清,采用酶联免疫吸附法(ELISA)测定TNF-α、IL-1β、IL-6水平,采用硝酸还原法测定NO水平。结果观察组血清TNF-α、IL-1β、IL-6水平高于对照组,而NO水平低于对照组,差异均有统计学意义(P<0.05);结肠癌患者和直肠癌患者血清TNF-α、NO、IL-1β、IL-6水平比较,差异无统计学意义(P>0.05);Ⅰ~Ⅱ期患者血清TNF-α、IL-1β、IL-6水平低于Ⅲ~Ⅳ期,而NO水平高于Ⅲ~Ⅳ期,差异均有统计学意义(P<0.05);手术后患者血清TNF-α、IL-1β、IL-6水平低于手术前,而NO水平高于手术前,差异均有统计学意义(P<0.05)。结论结直肠癌患者血清TNF-α、IL-1β、IL-6水平随着病情加重其水平升高,而NO水平随着病情加重其水平降低。

Objective To investigate the clinical significance the clinical significance of the changes of serum tumor necrosis factor-α(TNF-α),nitric oxide(NO),interleukin-1β(IL-1β),and interleukin-6(IL-6)in patients with colorectal cancer during perioperative period.Methods A total of 73 patients with colorectal cancer who underwent radical surgery in our hospital during December 2014 to December 2016 were selected as the observation group.At the same time,42 cases of healthy persons in our hospital from December 2014 to December 2016 were selected as control group.The 5 mL of peripheral venous blood was collected 2 days after admission and 3 days after admission,and serum was separated.The levels of TNF-,IL-1 and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA).The level of NO was measured by nitric acid reduction method(mL).Results The serum levels of TNF-α,IL-1β,IL-6 content in the observation group were higher than those in the control group,and the content of NO was lower than that of control group,and the difference was statistically significant(P<0.05);there were no significant differences between the serum TNF-α,NO,IL-1β,IL-6 levels of colon and rectal cancer patients(P>0.05);TNF-α,IL-1β,IL-6 levels of patients at I and II phase were lower than those of patients at III and IV phase,while the content of NO was higher than that of patients at III and IV phase,and the difference was statistically significant(P<0.05);serum TNF-α,IL-1β,IL-6 levels after the operation were lower than those before operation,and the content of NO was higher than that before operation,and the difference was statistically significant(P<0.05).Conclusion The levels of serum TNF-a,IL-1 beta and IL-6 in the patients with colorectal cancer were increased with the severity of the disease,while the NO decreased with the severity of the disease.