高效液相色谱法测定山楂黄酮的研究

Determination of flavonoids in hawthorn by HPLC

对高效液相色谱测定山楂中主要黄酮类物质的方法进行了研究,建立了定性定量测定牡荆素、芦丁和槲皮素的方法。方法学验证结果表明,重复性试验相对标准偏差(RSD)为1.73%,稳定性试验相对标准偏差(RSD)为1.04%,加标回收率为99.63%~104.80%。对5个不同地区的山楂样品进行检测,其样品与对照色谱图谱相似度在0.772~0.993之间,说明不同地区山楂中的黄酮组成存在较大差别。5个不同地区山楂中牡荆素、芦丁、槲皮素3种主要山楂黄酮的总量在420.47~897.80 mg/kg之间,其中芦丁含量为产生差别的主要原因。

A method for the determination of main flavonoids in hawthorn by H PLC was studied,and the qualitative and quantitative method for vitexin,rutin and quercetin determination was established.Methodological validation results showed that the relative standard deviation(RSD)of repeatability tests was 1.73%,the RSD of stability tests was 1.04%.The adding standard recovery rate was 99.63%-104.80%.The samples of hawthorn i^om five different regions were tested,and the similarity of chromatogram between sample and control was between 0.772-0.993.It showed that there were big differences in flavonoids composition in hawthorn from different regions.The total amount of three main hawthorn flavonoids vitexin,rutin,quercetin were between 420.47-897.80 mg/kg.The content of rutin was the main reason for the difference.