盘龙参组织培养与快繁研究

Tissue Culture and Rapid Propagation of Spiranthes sinensis

以野生花卉植物盘龙参Spiranthes sinensis的芽、花序为外植体,探讨不同消毒处理及植物生长调节剂对盘龙参组织培养的影响,建立组织培养快繁体系。结果显示,以0.1%升汞消毒6 min处理效果较好;丛生芽形成、花序愈伤组织诱导最适合的培养基组合为1/2MS+6-BA 2.5 mg·L^(-1)+NAA 0.25 mg·L^(-1);最佳增殖培养基组合为1/2MS+6-BA 2.0 mg·L^(-1)+NAA 0.75 mg·L^(-1);最佳生根培养基为1/2MS+NAA 1.25 mg·L^(-1)。

The effects of different disinfection treatment and the combinations of plant growth regulators on Spiranthes sinensis tissue culture with bud and inflorescence as explants were studied,and the rapid propagation system for tissue culture was established.The results indicated that the appropriate method for disinfection treatment on explants was in 0.1%mercuric chloride for 6 min.The optimal induction medium for multiple buds formation and callus induction was 1/2MS+6-BA 2.5 mg·L-1+NAA 0.25 mg·L-1.The optimum medium for proliferation was 1/2MS+6-BA 2.0 mg·L-1+NAA 0.75 mg·L-1.The optimum rooting medium was 1/2MS+NAA 1.25 mg·L-1.