麦门冬汤对肺纤维化模型大鼠BMSCs动员的影响

Effect of Maimendong decoction on BMSCs mobilization in model rats with pulmonary fibrosis

目的:观察麦门冬汤对肺纤维化模型大鼠BMSCs细胞动员的影响。方法:雌性SD大鼠40只随机分为4组。A组(空白组):予蒸馏水按1 m L/100 g灌胃;B组(模型组):造模24 h后,予蒸馏水按1 m L/100 g灌胃;C组(麦门冬汤组):造模24 h后,予麦门冬汤按生药1 g/m L/100 g灌胃(每毫升合生药1.1 g,相当于成人有效剂量),每日1次,连续7天;D组(西药对照组):造模后,按10μg/(kg·d)剂量皮下注射PBS稀释的rh G-CSF注射液,隔天注射1次,共4次。于建模后的第7天处死大鼠,流式细胞仪检测各组外周血中CD90、CD44阳性细胞表达的数量。结果:与空白组比较,模型组、麦门冬汤组、西药对照组外周血CD44、CD90细胞数均明显增加(P<0.05)。与模型组比较,麦门冬汤组、西药对照组外周血CD44、CD90细胞数均明显增加(P<0.05)。与西药对照组比较,麦门冬汤组外周血CD44、CD90细胞数无明显增加,差异无统计学意义(P>0.05)。结论:麦门冬汤通过对肺纤维化模型大鼠BMSCs细胞动员促进外周血CD90、CD44数量阳性细胞的表达,该作用可能是改善肺纤维化的机制之一。

Objective:To observe the effect of Maimendong decoction on BMSCs cell mobilization in model rats with pulmonary fibrosis.Methods:40 female SD rats were randomly divided into 4 groups.A group(blank group):1 mL/100 g distilled water by gavage;B group(model group):after 24 h of modeling,1 mL/100 g distilled water by gavage;C group(Maimendong decoction group):after 24 h of modeling,1 g/mL/100 g Maimendong decoction(crude drug,equivalent to adult effective dose)by gavage,once daily,continuously for 7 days;D group(western medicine control group):after modeling,rhG-CSF injection(diluted by PBS),according to 10μg/(kg-d)dose,by subcutaneous injection,once injection every other day,a total of 4 times.The rats were sacrificed on the 7 th day after modeling.The number of CD90 and CD44 positive cells expressed in peripheral blood of each group was detected by flow cytometry.Results:Compared with A group,the number of CD44 and CD90 cells in peripheral blood of B,C and D group was significantly increased(P<0.05).Compared with B group,the number of CD44 and CD90 cells in peripheral blood of C and D group was significantly increased(P<0.05).Compared with D group,the number of CD44 and CD90 cells in peripheral blood of C group was not significantly increased,the difference was not statistically significant(P>0.05).Conclusion:Through BMSCs cell mobilization in model rats with pulmonary fibrosis,Maimendong decoction promotes the number of CD44 and CD90 positive cells expressed in peripheral blood,which may be one of mechanisms to improve pulmonary fibrosis.