抗菌肽对镇海林蛙蝌蚪生长发育的影响

Effects of Antimicrobial Peptides on Growth and Development of Rana zhenhaiensis Tadpoles

本试验旨在探讨饲料中添加不同浓度抗菌肽对镇海林蛙(Rana zhenhaiensis)蝌蚪生长发育的影响。选取镇海林蛙蝌蚪(Gosner 26~27期)500只,随机分为5个组,每组5个重复,每个重复20尾。各组分别喂添加0、1、2、4和8 mg/g抗菌肽的试验饲料。试验至镇海林蛙蝌蚪全部完全变态结束。结果表明:1)抗菌肽浓度与镇海林蛙蝌蚪的发育历期呈显著的负相关(P<0.001),与镇海林蛙蝌蚪的体长相关性不显著(P=0.324);2)镇海林蛙蝌蚪的变态率平均值为94.6%,组间差异不显著(P=0.549),与抗菌肽浓度的相关性不显著(P=0.434);3)抗菌肽浓度与镇海林蛙蝌蚪的变态时间及体长、体重均呈显著正相关(P<0.001);4)抗菌肽浓度与镇海林蛙变态幼蛙的跳跃距离相关性不显著(P=0.578);5)抗菌肽浓度与镇海林蛙变态幼蛙大腿肌肉的超氧化物歧化酶活性呈显著负相关(P<0.001)。由此可见,抗菌肽的添加降低了镇海林蛙的发育速度,延长了变态时间,同时增加了变态个体的大小,从而提高了幼蛙的适合度。

This experiment was conducted to investigate the effects of antimicrobial peptides on growth and development of Rana zhenhaiensis tadpoles.Five hundred Rana zhenhaiensis tadpoles(Gosner 26 to 27 stages)were randomly allocated to 5 groups with 5 replicates per group and 20 tails per replicate.Tadpoles were fed the basic diet supplemented with 0,1,2,4 and 8 mg/g antimicrobial peptides,respectively.The experiment ended when Rana zhenhaiensis tadpoles complete metamorphosis.The results as showed as follows:1)the concentration of antimicrobial peptides was significantly negatively correlated with the developmental stage of Rana zhenhaiensis tadpoles(P<0.001),but had no significant correlation with body length of Rana zhenhaiensis tadpoles(P=0.324).2)The average value of metamorphic rate of Rana zhenhaiensis tadpoles was 94.6%,there were no significant differences among all groups(P=0.549),and had no significant correlation with the concentration of antimicrobial peptides(P=0.434).3)The metamorphic time,body length and body mass of Rana zhenhaiensis tadpoles were significantly positively correlated with the concentration of antimicrobial peptides(P<0.001).4)The jumping distance of Rana zhenhaiens abnormal froglets had no significant correlation with the concentration of antimicrobial peptides(P=0.578).5)The concentration of antimicrobial peptides was significantly negatively correlated with the thigh muscle superoxide dismutase activity of Rana zhenhaiens abnormal froglets(P<0.001).These findings showed that antimicrobial peptides can slow down the development rate of Rana zhenhaiens,prolong the metamorphic time,and form larger the size at metamorphosis,thus improve the fitness of froglets.